[Emergent viruses: SARS-associate coronavirus and H5N1 influenza virus].
Identifieur interne : 006692 ( Main/Exploration ); précédent : 006691; suivant : 006693[Emergent viruses: SARS-associate coronavirus and H5N1 influenza virus].
Auteurs : A. Goffard [France] ; M. Lazrek ; C. Schanen ; P-E Lobert ; L. Bocket ; A. Dewilde ; D. HoberSource :
- Annales de biologie clinique [ 0003-3898 ]
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Grippe humaine, Syndrome respiratoire aigu sévère.
- pathogénicité : Sous-type H5N1 du virus de la grippe A, Virus du SRAS.
- épidémiologie : Grippe humaine, Syndrome respiratoire aigu sévère.
- Humains, Maladies transmissibles émergentes.
English descriptors
- KwdEn :
- MESH :
- diagnosis : Influenza, Human, Severe Acute Respiratory Syndrome.
- epidemiology : Influenza, Human, Severe Acute Respiratory Syndrome.
- pathogenicity : Influenza A Virus, H5N1 Subtype, SARS Virus.
- Communicable Diseases, Emerging, Humans.
Abstract
Two viral agents with RNA genome are responsible for emerging illnesses: influenza virus A/H5N1 and Severe Acute Respiratory Syndrome virus (SARS). For the diagnosis of SARS virus infection, an epidemiological investigation is necessary to know whether the patient has been exposed to a risk in a country where the SARS virus is circulating or whether the patient had worked in a laboratory handling SARS virus. The detection of SARS virus is possible in various clinical samples (including urine) by viral culture or RT-PCR. The handling of those samples and RNA extraction must be performed in a BSL3 laboratory. The SARS virus RT-PCR is poorly sensitive, therefore the test should be performed on samples collected consecutively for several days. In front of a suspicion of A/H5N1, similar procedures are recommended. An epidemiologic investigation is necessary to specify whether the patient stayed in a country where A/H5N1 virus was circulating. Clinical samples needed for a specific diagnosis are: nasopharyngeal, throat-swab or fecal samples, cerebrospinal fluid and blood. The presence of A/H5N1 virus is confirmed by viral isolation or RNA detection by RT-PCR. RNA extraction must be performed in a BSL3 laboratory. For diagnosis of A/H5N1 virus infection, RT-PCR test amplifies specifically a fragment of H5 gene (Hemagglutinin). In french laboratories of medical virology, procedures are ready to diagnose the first case of A/H5N1 virus infection and cases of reemerging SARS virus infection.
PubMed: 16698555
Affiliations:
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Le document en format XML
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Schanen</name></author><author><name sortKey="Lobert, P E" sort="Lobert, P E" uniqKey="Lobert P" first="P-E" last="Lobert">P-E Lobert</name></author><author><name sortKey="Bocket, L" sort="Bocket, L" uniqKey="Bocket L" first="L" last="Bocket">L. Bocket</name></author><author><name sortKey="Dewilde, A" sort="Dewilde, A" uniqKey="Dewilde A" first="A" last="Dewilde">A. Dewilde</name></author><author><name sortKey="Hober, D" sort="Hober, D" uniqKey="Hober D" first="D" last="Hober">D. 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Goffard</name><affiliation wicri:level="4"><nlm:affiliation>Service de virologie, UPRES-EA3610, CHRU Lille, Faculté de Médecine, Université Lille 2, Bâtiment P. Boulanger, Lille.</nlm:affiliation><orgName type="university">Université Lille 2</orgName><country>France</country><placeName><settlement type="city">Lille</settlement><region type="region" nuts="2">Hauts-de-France</region><region type="old region" nuts="2">Nord-Pas-de-Calais</region></placeName></affiliation></author><author><name sortKey="Lazrek, M" sort="Lazrek, M" uniqKey="Lazrek M" first="M" last="Lazrek">M. Lazrek</name></author><author><name sortKey="Schanen, C" sort="Schanen, C" uniqKey="Schanen C" first="C" last="Schanen">C. Schanen</name></author><author><name sortKey="Lobert, P E" sort="Lobert, P E" uniqKey="Lobert P" first="P-E" last="Lobert">P-E Lobert</name></author><author><name sortKey="Bocket, L" sort="Bocket, L" uniqKey="Bocket L" first="L" last="Bocket">L. Bocket</name></author><author><name sortKey="Dewilde, A" sort="Dewilde, A" uniqKey="Dewilde A" first="A" last="Dewilde">A. Dewilde</name></author><author><name sortKey="Hober, D" sort="Hober, D" uniqKey="Hober D" first="D" last="Hober">D. Hober</name></author></analytic><series><title level="j">Annales de biologie clinique</title><idno type="ISSN">0003-3898</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Humans</term><term>Influenza A Virus, H5N1 Subtype (pathogenicity)</term><term>Influenza, Human (diagnosis)</term><term>Influenza, Human (epidemiology)</term><term>SARS Virus (pathogenicity)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Grippe humaine (diagnostic)</term><term>Grippe humaine (épidémiologie)</term><term>Humains</term><term>Maladies transmissibles émergentes</term><term>Sous-type H5N1 du virus de la grippe A (pathogénicité)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Grippe humaine</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Communicable Diseases, Emerging</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Maladies transmissibles émergentes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Two viral agents with RNA genome are responsible for emerging illnesses: influenza virus A/H5N1 and Severe Acute Respiratory Syndrome virus (SARS). For the diagnosis of SARS virus infection, an epidemiological investigation is necessary to know whether the patient has been exposed to a risk in a country where the SARS virus is circulating or whether the patient had worked in a laboratory handling SARS virus. The detection of SARS virus is possible in various clinical samples (including urine) by viral culture or RT-PCR. The handling of those samples and RNA extraction must be performed in a BSL3 laboratory. The SARS virus RT-PCR is poorly sensitive, therefore the test should be performed on samples collected consecutively for several days. In front of a suspicion of A/H5N1, similar procedures are recommended. An epidemiologic investigation is necessary to specify whether the patient stayed in a country where A/H5N1 virus was circulating. Clinical samples needed for a specific diagnosis are: nasopharyngeal, throat-swab or fecal samples, cerebrospinal fluid and blood. The presence of A/H5N1 virus is confirmed by viral isolation or RNA detection by RT-PCR. RNA extraction must be performed in a BSL3 laboratory. For diagnosis of A/H5N1 virus infection, RT-PCR test amplifies specifically a fragment of H5 gene (Hemagglutinin). In french laboratories of medical virology, procedures are ready to diagnose the first case of A/H5N1 virus infection and cases of reemerging SARS virus infection.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Hauts-de-France</li><li>Nord-Pas-de-Calais</li></region><settlement><li>Lille</li></settlement><orgName><li>Université Lille 2</li></orgName></list><tree><noCountry><name sortKey="Bocket, L" sort="Bocket, L" uniqKey="Bocket L" first="L" last="Bocket">L. Bocket</name><name sortKey="Dewilde, A" sort="Dewilde, A" uniqKey="Dewilde A" first="A" last="Dewilde">A. Dewilde</name><name sortKey="Hober, D" sort="Hober, D" uniqKey="Hober D" first="D" last="Hober">D. Hober</name><name sortKey="Lazrek, M" sort="Lazrek, M" uniqKey="Lazrek M" first="M" last="Lazrek">M. Lazrek</name><name sortKey="Lobert, P E" sort="Lobert, P E" uniqKey="Lobert P" first="P-E" last="Lobert">P-E Lobert</name><name sortKey="Schanen, C" sort="Schanen, C" uniqKey="Schanen C" first="C" last="Schanen">C. Schanen</name></noCountry><country name="France"><region name="Hauts-de-France"><name sortKey="Goffard, A" sort="Goffard, A" uniqKey="Goffard A" first="A" last="Goffard">A. Goffard</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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